Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

PCR Detection of Escherichia coli O157:H7 Directly from Stools: Evaluation of Commercial Extraction Methods for Purifying Fecal DNA

1 Nov 2000

Abstract excerpt

Rapid identification of Escherichia coli O157:H7 is important for patient management and for prompt epidemiological investigations. We evaluated one in-house method and three commercially available kits for their ability to extract E. coli O157:H7 DNA directly from stool specimens for PCR. Of the 153 stool specimens tested, 107 were culture positive and 46 were culture negative. The sensitivities and...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Escherichia coli research studies
Share to Salmonella and Campylobacter epidemiology
Share to Viral gastroenteritis research and epidemiology

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 11060076
DOI: 10.1128/jcm.38.11.4108-4113.2000

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

PCR Detection of Escherichia coli O157:H7 Directly from Stools: Evaluation of Commercial Extraction Methods for Purifying Fecal DNAPMID 11060076Read ↗

A comparison of the efficiency of five different commercial DNA extraction kits for extraction of DNA from faecal samplesSemantic proximity Read ↗

Microbial diversity in fecal samples depends on DNA extraction method: easyMag DNA extraction compared to QIAamp DNA stool mini kit extractionSemantic proximity Read ↗

Methods for Detecting Enterohaemorrhagic Escherichia Coli in FoodSemantic proximity Read ↗

Direct Isolation of DNA from Patient Stools for Polymerase Chain Reaction Detection ofCryptosporidium parvumSemantic proximity Read ↗

A comparison of two approaches to extracting Cryptosporidium DNA from human stools as measured by a real-time PCR assaySemantic proximity Read ↗

Detection of human enterovirus and human parechovirus (HPeV) genotypes from clinical stool samples: polymerase chain reaction and direct molecular typing, culture characteristics, and serotyping.Semantic proximity Read ↗

A Simple Inexpensive Alkaline Method for DNA Extraction from Environmental Samples for PCR SurveillanceSemantic proximity Read ↗

Molecular characterization of the gene encoding H antigen in Escherichia coli and development of a PCR-restriction fragment length polymorphism test for identification of E. coli O157:H7 and O157:NMSemantic proximity Read ↗

Multiplex PCR for identification of Campylobacter coli and Campylobacter jejuni from pure cultures and directly on stool samplesSemantic proximity Read ↗

A method for preparation of fecal DNA suitable for PCRSemantic proximity Read ↗

A simple polymerase chain reaction technique to detect and differentiate Shigella and enteroinvasive escherichia coli in human fecesSemantic proximity Read ↗

New Methods for Detection of Campylobacters in Stool Samples in Comparison to CultureSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.