Article
Polymerization, three-dimensional structure and mechanical properties of Ddictyostelium versus rabbit muscle actin filaments.
Journal of molecular biology - 20 Oct 2000
Steinmetz M O, Hoenger A, Stoffler D, Noegel A A, Aebi U, Schoenenberger C A
Abstract excerpt
To assess more systematically functional differences among non-muscle and muscle actins and the effect of specific mutations on their function, we compared actin from Dictyostelium discoideum (D-actin) with actin from rabbit skeletal muscle (R-actin) with respect to the formation of filaments, their three-dimensional structure and mechanical properties. With Mg(2+) occupying the single high-affinity divalent...
Topics
- Actin Cytoskeleton
- Actins
- Animals
- Binding Sites
- Biopolymers
- Calcium
- Cations, Divalent
- Dictyostelium
- Fluorescence
- Gadolinium
- Image Processing, Computer-Assisted
