Article
Evidence of an efflux pump in Serratia marcescens.
Microbial drug resistance (Larchmont, N.Y.) - 1 Jan 2000
Berlanga M, Vázquez J L, Hernandez-Borrell J, Montero M T, Viñas M
Abstract excerpt
Spontaneous mutants resistant to fluoroquinolones were obtained by exposing Serratia marcescens NIMA (wild-type strain) to increasing concentrations of ciprofloxacin both in liquid and on solid media. Frequencies of mutation ranged from 10(-7) to 10(-9). Active expulsion of antibiotic was explored as a possible mechanism of resistance in mutants as well as changes in topoisomerase target genes. The role of...
Topics
- Anti-Bacterial Agents
- Anti-Infective Agents
- Bacterial Outer Membrane Proteins
- Base Sequence
- Cell Membrane Permeability
- Ciprofloxacin
- Culture Media
- DNA Gyrase
- DNA Topoisomerases, Type II
- Drug Resistance, Microbial
- Drug Resistance, Multiple
- Humans
- Lipopolysaccharides
- Microbial Sensitivity Tests
- Molecular Sequence Data
- Mutation
- Serratia marcescens