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Quantitative analysis of human DNA sequences by PCR and solid-phase minisequencing.

Molecular biotechnology - 1 Jun 2000

Suomalainen A, Syvänen A C

Abstract excerpt

Reliable quantification by PCR requires careful experimental design and conditions, often involving sampling of the PCR reactions at different time points or amplifying multiple dilutions of a standard DNA. We describe here an accurate, quantitative and easily automatizable solid-phase method based on competitive PCR. The PCR products are analyzed by solid-phase mini-sequencing after capture of biotinylated PCR...

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PMID: 10949825
DOI: 10.1385/MB:15:2:123

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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