Article
An extended conformation of calmodulin induces interactions between the structural domains of adenylyl cyclase from Bacillus anthracis to promote catalysis.
The Journal of biological chemistry - 17 Nov 2000
Drum C L, Yan S Z, Sarac R, Mabuchi Y, Beckingham K, Bohm A, Grabarek Z, Tang W J
Abstract excerpt
The edema factor exotoxin produced by Bacillus anthracis is an adenylyl cyclase that is activated by calmodulin (CaM) at resting state calcium concentrations in infected cells. A C-terminal 60-kDa fragment corresponding to the catalytic domain of edema factor (EF3) was cloned, overexpressed in Escherichia coli, and purified. The N-terminal 43-kDa domain (EF3-N) of EF3, the sole domain of edema factor homologous...
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