Article
High-throughput in planta expression screening identifies an ADP-ribosylation factor (ARF1) involved in non-host resistance and R gene-mediated resistance.
Molecular plant pathology - 1 Jan 2008
Coemans Bert, Takahashi Yoshihiro, Berberich Thomas, Ito Akiko, Kanzaki Hiroyuki, Matsumura Hideo, Saitoh Hiromasa, Tsuda Shinya, Kamoun Sophien, Sági László, Swennen Rony, Terauchi Ryohei
Abstract excerpt
To identify positive regulators of cell death in plants, we performed a high-throughput screening, employing potato virus X-based overexpression in planta of a cDNA library derived from paraquat-treated Nicotiana benthamiana leaves. The screening of 30,000 cDNA clones enabled the identification of an ADP-ribosylation factor 1 (ARF1) that induces cell death when overexpressed in N. benthamiana. Overexpression of...
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