Article
Oka varicella vaccine is distinguishable from its parental virus in DNA sequence of open reading frame 62 and its transactivation activity.
Journal of medical virology - 1 Aug 2000
Gomi Y, Imagawa T, Takahashi M, Yamanishi K
Abstract excerpt
When the nucleotide sequences of the Oka vaccine and its parental varicella-zoster virus (VZV) were compared in 6 open reading frames (ORFs), glycoprotein C (gC) and 5 transactivator genes, mutations were detected only in the immediate-early gene 62. The vaccine virus contained a mixture of different sequences that had variations at 15 nucleotide positions, but only one sequence was found for the Oka parental...
Topics
- Cells, Cultured
- Chickenpox Vaccine
- DNA Primers
- Genes, Immediate-Early
- Glycoproteins
- Herpesvirus 3, Human
- Humans
- Mutation
- Open Reading Frames
- Polymorphism, Restriction Fragment Length
- Promoter Regions, Genetic
- Transcriptional Activation
- Transfection
- Vaccines, Attenuated
- Viral Envelope Proteins