Article
Characterization of the Sinorhizobium meliloti genes encoding a functional dihydrodipicolinate synthase (dapA) and dihydrodipicolinate reductase (dapB).
Archives of microbiology - 1 Jan 2000
García-Rodríguez F M, Zekri S, Toro N
Abstract excerpt
In bacteria, the known pathways for diaminopimelate (DAP) and lysine biosynthesis share two key enzymes, dihydrodipicolinate synthase and dihydrodipicolinate reductase, encoded by the dapA and dapB genes, respectively. In rhizobia, these genes have not yet been genetically characterized. In this work, by sequence analysis, we identified two divergent open reading frames on the 140-MDa plasmid pRmeGR4b of...
Topics
- Amino Acid Sequence
- Consensus Sequence
- Dihydrodipicolinate Reductase
- Escherichia coli
- Genes, Bacterial
- Genetic Complementation Test
- Hydro-Lyases
- Molecular Sequence Data
- Oxidoreductases
- Oxidoreductases Acting on CH-CH Group Donors
- Phenotype
- Plasmids
- Reverse Transcriptase Polymerase Chain Reaction