Article
A unique second donor splice site in the intron 5 sequence of the HLA-A*11 alleles results in a class I transcript encoding a molecule with an elongated cytoplasmic domain.
Tissue antigens - 1 May 2000
Tijssen H J, Sistermans E A, Joosten I
Abstract excerpt
Full-length cDNA sequencing of the A*1103 allele revealed an insertion of 18 bp between exon 5 and 6. We hypothesized that this could be the result of alternative splicing. Sequencing of intron-5 of A*1101, *1102 and *1103 alleles demonstrated that this 18-bp insertion consisted of the 5'-end of intron 5, concluded by a second in-frame donor splice site. Alignment of the 5'-end intron 5 sequence of A*1101-3 with...
Topics
- Alleles
- Alternative Splicing
- Amino Acid Sequence
- B-Lymphocytes
- Cell Line, Transformed
- Cytoplasm
- Female
- Gene Expression Regulation
- HLA-A Antigens
- HLA-A11 Antigen
- Humans
- Introns
- Molecular Sequence Data
- Protein Biosynthesis
- Protein Structure, Tertiary
- RNA Splice Sites
- RNA, Messenger
- Transcription, Genetic