Article
A solid phase plate assay for HIV-1 genotyping.
Molecular and cellular probes - 1 Jun 2000
González-Villaseñor L I, Wu K, Yang L
Abstract excerpt
A prototype solid phase plate assay (SPPA) for the detection and genotyping of HIV-1 subtypes was developed using a PCR-based capture hybridization format. Well characterized HIV-1 reference controls of known subtypes and subtype specific capture oligonucleotide probes targeting several regions of the envelope (env) gene of HIV-1 were selected to develop the assay. The subtype specific oligonucleotide probes were...
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