Article
Distinguishing molecular forms of HIV-1 in Asia with a high-throughput, fluorescent genotyping assay, MHAbce v.2.
Virology - 5 Feb 2007
Kijak Gustavo H, Tovanabutra Sodsai, Sanders-Buell Eric, Watanaveeradej Veerachai, de Souza Mark S, Nelson Kenrad E, Ketsararat Vidhaya, Gulgolgarn Vilawan, Wera-arpachai Manu, Sriplienchan Somchai, Khamboonrueng Chirasak, Birx Deborah L, Robb Merlin L, McCutchan Francine E
Abstract excerpt
High-resolution HIV-1 genotyping of large sample sets is crucial to define the evolving and dynamic epidemics in Asia. Here we present MHAbce v.2, a multi-region hybridization assay that individually discriminates subtypes B, C, CRF01_AE, and virtually all of their described recombinants, based on real-time PCR using subtype-specific TaqMan probes in 8 regions throughout the viral genome. In a validation panel...
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