Article
Molecular assessment of post-BMT chimerism using various biologic specimens and automated DNA sizing technology.
Journal of hematotherapy & stem cell research - 1 Apr 2000
Jółkowska J, Wachowiak J, Lange A, Kwissa M, Witt M
Abstract excerpt
Highly polymorphic microsatellite markers provide useful genetic markers for detection of complete or mixed chimerism in patients after allogeneic BMT (allo-BMT). We report application of automated DNA sizing technology for detection of post-BMT chimerism using fresh peripheral blood, BM, or archival blood smears and various DNA isolation techniques. Donors' and recipients' DNA was amplified with fluorescent PCR...
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