Article
Cloning and characterization of the lactate-specific inducible gene KlCYB2, encoding the cytochrome b(2) of Kluyveromyces lactis.
Yeast (Chichester, England) - 1 May 2000
Alberti A, Goffrini P, Ferrero I, Lodi T
Abstract excerpt
In yeast the utilization of lactate requires two enzymes, the D and L-lactate ferricytochrome c oxidoreductase (D and L-LCR), which stereospecifically oxidize D- and L-lactate to pyruvate. These enzymes are nuclearly encoded and localized in mitochondria. In the yeast Kluyveromyces lactis, a mutant devoid of D- and L-LCR activities and unable to grow on racemic lactate was isolated. Transformation of the mutant...
Topics
- Amino Acid Sequence
- Carbon
- Cloning, Molecular
- Gene Deletion
- Gene Expression Regulation, Fungal
- Genetic Complementation Test
- Kluyveromyces
- L-Lactate Dehydrogenase
- L-Lactate Dehydrogenase (Cytochrome)
- Lactates