Article
Cloning of beta-1,3-glucanases expressed during Cichorium somatic embryogenesis.
Plant molecular biology - 1 Jan 2000
Helleboid S, Chapman A, Hendriks T, Inzé D, Vasseur J, Hilbert J L
Abstract excerpt
Three different beta-1,3-glucanase cDNA fragments, CG1, CG2 and CG3, were obtained by RT-PCR from RNA isolated from Cichorium hybrid '474' leaf fragments cultured for 11 days under somatic embryogenesis-inducing conditions. When expressed in Escherichia coli the proteins encoded by the three cDNAs were recognized by antibodies raised against 38 kDa extracellular beta-1,3-glucanases studied previously (Helleboid...
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