Article
A carrot somatic embryo mutant is rescued by chitinase.
The Plant cell - 1 Apr 1992
De Jong A J, Cordewener J, Lo Schiavo F, Terzi M, Vandekerckhove J, Van Kammen A, De Vries S C
Abstract excerpt
At the nonpermissive temperature, somatic embryogenesis of the temperature-sensitive (ts) carrot cell mutant ts11 does not proceed beyond the globular stage. This developmental arrest can be lifted by the addition of proteins secreted by wild-type cells to the culture medium. From this mixture of secreted proteins, a 32-kD glycoprotein, designated extracellular protein 3 (EP3), that allows completion of somatic...
Topics
- Amino Acid Sequence
- Chitinases
- Culture Techniques
- Electrophoresis, Polyacrylamide Gel
- Molecular Sequence Data
- Mutation
- Plants
- Sequence Homology, Nucleic Acid
- Temperature