Article
Characterization of chitinases able to rescue somatic embryos of the temperature-sensitive carrot variant ts 11.
Plant molecular biology - 1 Jun 1996
Kragh K M, Hendriks T, de Jong A J, Lo Schiavo F, Bucherna N, Højrup P, Mikkelsen J D, de Vries S C
Abstract excerpt
To characterize the acidic endochitinase EP3, able to rescue somatic embryos of the carrot cell line ts11, the enzyme was purified from the medium of wild-type suspension cultures. Peptide sequences, deduced amino acid sequences of corresponding PCR-generated cDNA clones, serological relation and...
Topics
- Amino Acid Sequence
- Base Sequence
- Cells, Cultured
- Chitinases
- Chromatography, DEAE-Cellulose
- Chromatography, Gel
- Cloning, Molecular
- DNA Primers
- DNA, Complementary
- Daucus carota
- Fabaceae
- Genetic Variation
- Isoenzymes
- Kinetics
- Molecular Sequence Data