Article
Characterization of chitinases able to rescue somatic embryos of the temperature-sensitive carrot variant ts 11.
Plant molecular biology - 1 Jun 1996
Kragh K M, Hendriks T, de Jong A J, Lo Schiavo F, Bucherna N, Højrup P, Mikkelsen J D, de Vries S C
Abstract excerpt
To characterize the acidic endochitinase EP3, able to rescue somatic embryos of the carrot cell line ts11, the enzyme was purified from the medium of wild-type suspension cultures. Peptide sequences, deduced amino acid sequences of corresponding PCR-generated cDNA clones, serological relation and biochemical properties showed that there were at least five closely related chitinases, four of which could be...
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