Article
Role of metal ions in the reaction catalyzed by L-ribulose-5-phosphate 4-epimerase.
Biochemistry - 25 Apr 2000
Lee L V, Poyner R R, Vu M V, Cleland W W
Abstract excerpt
H97N, H95N, and Y229F mutants of L-ribulose-5-phosphate 4-epimerase had 10, 1, and 0.1%, respectively, of the activity of the wild-type (WT) enzyme when activated by Zn(2+), the physiological activator. Co(2+) and Mn(2+) replaced Zn(2+) in Y229F and WT enzymes, although less effectively with the His mutants, while Mg(2+) was a poorly bound, weak activator. None of the other eight tyrosines mutated to...
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