Article
Indigenous plasmids in a production line of strains for penicillin G acylase derived from Escherichia coli W.
Folia microbiologica - 1 Jan 1999
Sobotková L, Grafková J, Stĕpánek V, Vacík T, Maresová H, Kyslík P
Abstract excerpt
Three indigenous plasmids designated pRK1, pRK2 and pRK3 were identified among producers of penicillin G acylase (PGA) derived from the strain Escherichia coli W ATCC 9637. Their size and copy number (CN) in E. coli W were determined (kb; CN): pRK1 (80; 3.4), pRK2 (5.1; 71), and pRK3 (4.8; 13.7). Strain E. coli RE2 harboring these plasmids was used for selection of strains with reduced number of plasmids: the...
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