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Article

Genetic diversity and the absence of regional differences of Borrelia garinii as demonstrated by ospA and ospB gene sequence analysis.

Microbiology and immunology - 1 Jan 1999

Yabuki M, Nakao M, Fukunaga M

Abstract excerpt

Unfed adult Ixodes persulcatus ticks were collected from four locations of Nagano and Hokkaido in Japan. Infected Borrelia garinii were investigated by PCR-RFLP of the ospA and ospB gene sequences. The primer set amplified an approximately 1.6-kb DNA fragment (0.7-kb in some strains), and BsrI, BstYI, or NlaIII digestion of the product resulted in six distinctively different PCR-RFLP groups and two independent...

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Identifiers

PMID: 10656177
DOI: 10.1111/j.1348-0421.1999.tb03366.x

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