Article
A bacterial high-affinity GABA binding protein: isolation and characterization.
Biochemical and biophysical research communications - 5 Feb 2000
Guthrie G D, Nicholson-Guthrie C S, Leary H L
Abstract excerpt
A gamma-aminobutyric acid (GABA) binding protein (GBP) was isolated from a bacterial mutant which has high-affinity GABA binding characteristics comparable with the GABA(A) brain receptor in mammals. The GBP was partially purified and characterized and was shown to be a periplasmic protein of approximately 42,000 molecular weight. To determine the molecular weight, a bacterial GABA binding assay was used with...
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