Article
Nutritional control of nucleocytoplasmic localization of cAMP-dependent protein kinase catalytic and regulatory subunits in Saccharomyces cerevisiae.
The Journal of biological chemistry - 14 Jan 2000
Griffioen G, Anghileri P, Imre E, Baroni M D, Ruis H
Abstract excerpt
In budding yeast, cAMP-dependent protein kinase (PKA) plays a central role in the nutritional control of metabolism, cell cycle, and transcription. This study shows that both the regulatory subunit Bcy1p and the catalytic subunit Tpk1p associated with it are predominantly localized in the nucleus of rapidly growing cells. Activation of nuclear PKA by cAMP leads to fast entry of a significant part of Tpk1p into...
Topics
- Cell Nucleus
- Cyclic AMP
- Cyclic AMP-Dependent Protein Kinases
- Cytoplasm
- Genotype
- Green Fluorescent Proteins
- Kinetics
- Luminescent Proteins
- Macromolecular Substances
- Plasmids
- Recombinant Fusion Proteins
- Saccharomyces cerevisiae
- Sequence Deletion