Article
Effect of branch frequency in Aspergillus oryzae on protein secretion and culture viscosity.
Biotechnology and bioengineering - 20 Dec 1999
Bocking S P, Wiebe M G, Robson G D, Hansen K, Christiansen L H, Trinci A P
Abstract excerpt
Highly branched mutants of two strains of Aspergillus oryzae (IFO4177, which produces alpha-amylase, and a transformant of IFO4177 [AMG#13], which produces heterologous glucoamylase in addition to alpha-amylase) were generated by UV or nitrous acid mutagenesis. Four mutants of the parental strain (IFO4177), which were 10 to 50% more branched than the parental strain, were studied in stirred batch culture and no...
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