Article
Branching mutants of Aspergillus oryzae with improved amylase and protease production on solid substrates.
Applied microbiology and biotechnology - 1 Nov 2005
te Biesebeke R, Record E, van Biezen N, Heerikhuisen M, Franken A, Punt P J, van den Hondel C A M J J
Abstract excerpt
To study the relation between the number of hyphal tips and protein secretion during growth on a solid substrate, we have constructed two mutant strains of Aspergillus oryzae with increased hyphal branching. We have analysed hydrolytic enzyme activities during growth on wheat kernels (WK) of A. oryzae strains carrying the disrupted allele of the pclA gene encoding a secretion pathway specific (KEX2-like)...
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