Article
Isolation of a potential neural stem cell line from the internal capsule of an adult transgenic rat brain.
Journal of neurochemistry - 1 Nov 1999
Kilty I C, Barraclough R, Schmidt G, Rudland P S
Abstract excerpt
A thermosensitive mutation of simian virus 40 large T antigen (LTA) gene, the tsA58 gene, was cloned downstream of the 6-kbp neurofilament light chain promoter in pPOLYIII and injected into the pronucleus of fertilised oocytes of Sprague-Dawley rats to develop a strain harbouring six copies of the transgene. Immunocytochemical staining of hemizygous adult tissues with antibodies to the C-terminus of LTA showed...
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