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Nuclear transfer in cattle with non‐transfected and transfected fetal or cloned transgenic fetal and postnatal fibroblasts

28 Sept 2001

Abstract excerpt

The efficiency of nuclear transfer (NT) using two primary cultures of fetal fibroblasts (FF1 and FF2) was compared vs. the same cultures transfected with an expression vector in which the bovine prochymosin coding sequence is placed under the control of the bovine alpha(S1)-casein promoter (TFF1 and TFF2). In addition, fibroblasts of a cloned transgenic fetus (TRFF1) derived from TFF1 and ear skin fibroblasts of...

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PMID: 11599047
DOI: 10.1002/mrd.1098

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