Article
Sigma factor displacement from RNA polymerase during Bacillus subtilis sporulation.
Journal of bacteriology - 1 Aug 1999
Ju J, Mitchell T, Peters H, Haldenwang W G
Abstract excerpt
As Bacillus subtilis proceeds through sporulation, the principal vegetative cell sigma subunit (sigma(A)) persists in the cell but is replaced in the extractable RNA polymerase (RNAP) by sporulation-specific sigma factors. To explore how this holoenzyme changeover might occur, velocity centrifugation techniques were used in conjunction with Western blot analyses to monitor the associations of RNAP with sigma(A)...
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