Article
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry analysis of zeins in mature maize kernels.
Journal of agricultural and food chemistry - 7 Apr 2004
Adams Whitney R, Huang Shihshieh, Kriz Alan L, Luethy Michael H
Abstract excerpt
A high-throughput method has been developed to allow rapid analysis of maize seed storage proteins by matrix-assisted laser desorption time-of-flight mass spectrometry. The extraction solution containing an organic solvent, a reducing agent, and a volatile base has been optimized to enable extraction of all classes of zein proteins (alpha-, beta-, gamma-, and delta-). A near-saturating concentration of matrix,...
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