Article
Quantification of individual zein isoforms resolved by two-dimensional electrophoresis: genetic variability in 45 maize inbred lines.
Electrophoresis - 1 Aug 2001
Consoli L, Damerval C
Abstract excerpt
A two-dimensional (2-D) electrophoresis procedure was optimized to obtain well-resolved and reproducible patterns of zein polypeptides in maize. All zein isoforms obtained through zein-specific extraction were observed from whole meal extracted with a urea/Triton/2-mercaptoethanol solution. Loading the protein samples at the acidic side of the gradient, using an amino acid solution as catholyte and running for a...
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