Article
Stable transfection of human fetal chondrocytes with a type II procollagen minigene: expression of the mutant protein and alterations in the structure of the extracellular matrix in vitro.
Arthritis and rheumatism - 1 Jul 1999
Dharmavaram R M, Liu G, Tuan R S, Stokes D G, Jiménez S A
Abstract excerpt
OBJECTIVE: To perform stable transfections of human chondrocytes under conditions that allow the maintenance of the chondrocyte-specific phenotype, and to examine the effects of the stable transfection of a mutated type II procollagen gene (COL2A1) on the structure of the cartilaginous extracellular matrix produced in vitro. METHODS: A type II procollagen minigene that lacks exons 16-27 was stably transfected...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.