Article
Minicircle variable region probes for characterization of Leishmania (Viannia) species.
The Journal of parasitology - 1 Jun 1999
Fernandes O, Catanho M P, Segura I, Labrada L A, Derré R, Saravia N, Degrave W
Abstract excerpt
The minicircle molecules present in the kinetoplast DNA (kDNA) network constitute a particularly useful molecular tool because they are a multicopy target and present a variable region that differs among minicircle classes in the same network. Using the polymerase chain reaction (PCR) and a set of primers directed outwardly from the minicircle conserved region, it is possible to prepare molecular probes...
Topics
- Animals
- DNA Primers
- DNA, Kinetoplast
- Electrophoresis, Agar Gel
- Genetic Variation
- Leishmania
- Nucleic Acid Hybridization
- Polymerase Chain Reaction
- Species Specificity