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Article

Identification of the folate binding sites on the Escherichia coli T-protein of the glycine cleavage system.

The Journal of biological chemistry - 18 Jun 1999

Okamura-Ikeda K, Fujiwara K, Motokawa Y

Abstract excerpt

T-protein is a component of the glycine cleavage system and catalyzes the tetrahydrofolate-dependent reaction. To determine the folate-binding site on the enzyme, 14C-labeled methylenetetrahydropteroyltetraglutamate (5,10-CH2-H4PteGlu4) was enzymatically synthesized from methylenetetrahydrofolate (5, 10-CH2-H4folate) and [U-14C]glutamic acid and subjected to cross-linking with the recombinant Escherichia coli...

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PMID: 10364177
DOI: 10.1074/jbc.274.25.17471

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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