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Article

pSKAP/S: An expression vector for the production of single-chain Fv alkaline phosphatase fusion proteins.

Protein expression and purification - 1 Jun 1999

Griep R A, van Twisk C, Kerschbaumer R J, Harper K, Torrance L, Himmler G, van der Wolf J M, Schots A

Abstract excerpt

The vector pSKAP/S was constructed to enable overexpression of single-chain variable fragment antibody (scFv)-alkaline phosphatase fusion proteins. In pSKAP/S, the scFv were genetically fused to the mutated Escherichia coli PhoA/S gene that encodes an alkaline phosphatase with increased specific activity. The restriction sites incorporated into pSKAP/S allowed the scFv genes to be easily transferred from...

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Source

Identifiers

PMID: 10336861
DOI: 10.1006/prep.1999.1041

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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