Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Role of tryptophanyl residues in tobacco acetolactate synthase.

Biochemical and biophysical research communications - 27 May 1999

Chong C K, Shin H J, Chang S I, Choi J D

Abstract excerpt

Acetolactate synthase (ALS) catalyzes the first common step in the biosynthesis of valine, leucine, and isoleucine. ALS is the target of three classes of herbicides, the sulfonylureas, the imidazolinones, and the triazolopyrimidines. Five mutants (W266F, W439F, W490F, W503F, and W573F) of the ALS gene from Nicotiana tabacum were constructed and expressed in Escherichia coli, and the enzymes were purified. The...

Topics

Acetolactate Synthase
Escherichia coli
Kinetics
Mutagenesis, Site-Directed
Mutation
Plants, Toxic
Recombinant Fusion Proteins
Spectrometry, Fluorescence
Spectrophotometry
Nicotiana
Tryptophan

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 10334929
DOI: 10.1006/bbrc.1999.0740

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-10334929-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Role of tryptophanyl residues in tobacco acetolactate synthase.PMID 10334929Read ↗

Roles of conserved methionine residues in tobacco acetolactate synthase.Semantic proximity Read ↗

Structural and functional evaluation of three well-conserved serine residues in tobacco acetohydroxyacid synthase.Semantic proximity Read ↗

Synthesis of the quinoline-linked triazolopyrimidine analogues and their interactions with the recombinant tobacco acetolactate synthase.Semantic proximity Read ↗

A novel Pro197Glu substitution in acetolactate synthase (ALS) confers broad-spectrum resistance across ALS inhibitors.Semantic proximity Read ↗

Unravelling the effect of two herbicide resistance mutations on acetolactate synthase kinetics and growth traits.Semantic proximity Read ↗

A new amino acid substitution (Ala-205-Phe) in acetolactate synthase (ALS) confers broad spectrum resistance to ALS-inhibiting herbicides.Semantic proximity Read ↗

Herbicide-resistant forms of Arabidopsis thaliana acetohydroxyacid synthase: characterization of the catalytic properties and sensitivity to inhibitors of four defined mutants.Semantic proximity Read ↗

An acetohydroxy acid synthase mutant reveals a single site involved in multiple herbicide resistance.Semantic proximity Read ↗

Selectable tolerance to herbicides by mutated acetolactate synthase genes integrated into the chloroplast genome of tobacco.Semantic proximity Read ↗

Resistance to acetolactate synthase inhibitors is due to a W 574 to L amino acid substitution in the ALS gene of redroot pigweed and tall waterhemp.Semantic proximity Read ↗

Acetolactate synthase mutation conferring imidazolinone-specific herbicide resistance in Amaranthus hybridus.Semantic proximity Read ↗

Molecular basis of multiple resistance to ACCase- and ALS-inhibiting herbicides in Alopecurus japonicus from China.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.