Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Accurate determination of the number of CAG repeats in the Huntington disease gene using a sequence-specific internal DNA standard.

Clinical genetics - 1 Mar 1999

Bruland O, Almqvist E W, Goldberg Y P, Boman H, Hayden M R, Knappskog P M

Abstract excerpt

We have developed a sequence-specific internal DNA size standard for the accurate determination of the number of CAG repeats in the Huntington disease (HD) gene by cloning key fragments (between 15 and 64 CAG repeats) of the HD gene. These fragments, pooled to produce a sequence-specific DNA ladder, enabled us to observe the true number of CAG repeats directly, with no need for calculations. Comparison of the...

Topics

Alleles
Autoradiography
Calibration
DNA
Humans
Huntingtin Protein
Huntington Disease
Isotope Labeling
Nerve Tissue Proteins
Nuclear Proteins
Reference Standards

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Trinucleotide Repeats

Source

Identifiers

PMID: 10334474
DOI: 10.1034/j.1399-0004.1999.550308.x

Genes

POP4

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Accurate determination of the number of CAG repeats in the Huntington disease gene using a sequence-specific internal DNA standard.PMID 10334474Read ↗

A worldwide study of the Huntington's disease mutation. The sensitivity and specificity of measuring CAG repeats.Semantic proximity Read ↗

Enhanced Detection and Sizing of the HTT CAG Repeat Expansion in Huntington Disease Using an Improved Triplet-Primed PCR Assay.Semantic proximity Read ↗

[Genetic diagnosis of Huntington disease].Semantic proximity Read ↗

Nonisotopic method for accurate detection of (CAG)n repeats causing Huntington disease.Semantic proximity Read ↗

Advances in Huntington's disease diagnostics: development of a standard reference material.Semantic proximity Read ↗

Evaluation of IP-RP-HPLC for length determination of the trinucleotide repeat fragments in Huntington's disease.Semantic proximity Read ↗

Accurate sizing of (CAG)n repeats causing Huntington disease by fluorescent PCR.Semantic proximity Read ↗

A Study of Triplet-Primed PCR for Identification of CAG Repeat Expansion in the HTT Gene in a Cohort of 503 Indian Cases with Huntington's Disease Symptoms.Semantic proximity Read ↗

A CCG repeat polymorphism adjacent to the CAG repeat in the Huntington disease gene: implications for diagnostic accuracy and predictive testing.Semantic proximity Read ↗

Triplet repeat primed PCR simplifies testing for Huntington disease.Semantic proximity Read ↗

Trinucleotide repeats in the human genome: size distributions for all possible triplets and detection of expanded disease alleles in a group of Huntington disease individuals by the repeat expansion detection method.Semantic proximity Read ↗

A Sau3A polymorphism in the 5' end of the IT15 gene that nonrandomly segregates with the Huntington disease trinucleotide expansion.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.