Article
Elucidation of a PTS-carbohydrate chemotactic signal pathway in Escherichia coli using a time-resolved behavioral assay.
Molecular biology of the cell - 1 Apr 1999
Lux R, Munasinghe V R, Castellano F, Lengeler J W, Corrie J E, Khan S
Abstract excerpt
Chemotaxis of Escherichia coli toward phosphotransferase systems (PTSs)-carbohydrates requires phosphoenolpyruvate-dependent PTSs as well as the chemotaxis response regulator CheY and its kinase, CheA. Responses initiated by flash photorelease of a PTS substrates D-glucose and its nonmetabolizable analog methyl alpha-D-glucopyranoside were measured with 33-ms time resolution using computer-assisted motion...
Topics
- Bacterial Proteins
- Chemotaxis
- Escherichia coli
- Escherichia coli Proteins
- Genotype
- Glucosides
- Histidine Kinase
- Kinetics
- Membrane Proteins
- Methyl-Accepting Chemotaxis Proteins
- Models, Chemical
