Article
pH-dependent spectroscopic changes associated with the hydroquinone of FMN in flavodoxins.
Biochemistry - 23 Mar 1999
Yalloway G N, Mayhew S G, Malthouse J P, Gallagher M E, Curley G P
Abstract excerpt
Photoreduction with a 5-deazaflavin as the catalyst was used to convert flavodoxins from Desulfovibrio vulgaris, Megasphaera elsdenii, Anabaena PCC 7119, and Azotobacter vinelandii to their hydroquinone forms. The optical spectra of the fully reduced flavodoxins were found to vary with pH in the pH range of 5.0-8.5. The changes correspond to apparent pKa values of 6.5 and 5.8 for flavodoxins from D. vulgaris and...
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