Article
Effects of substituting asparagine for glycine-61 in flavodoxin from Desulfovibrio vulgaris (Hildenborough).
Biochemistry international - 1 Jan 1990
Carr M C, Curley G P, Mayhew S G, Voordouw G
Abstract excerpt
Gly-61 in flavodoxin from Desulfovibrio vulgaris (Hildenborough) has been changed to Asn by site-directed mutagenesis of the cloned gene. Values determined for the dissociation constant for the dissociation of the mutant protein into apoprotein and FMN, and for the redox potentials of the two 1-electron steps in the reduction of the bound flavin showed that FMN in all three redox states is bound more weakly in...
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