Article
Cloning and sequencing of the Serratia marcescens gene encoding a single-stranded DNA-binding protein (SSB) and its promoter region.
Gene - 15 May 1993
de Vries J, Wackernagel W
Abstract excerpt
The gene (ssb) coding for a single-stranded DNA-binding protein (SSB) was identified on a 1.2-kb EcoRI-SalI fragment cloned from chromosomal DNA of Serratia marcescens. The cloned fragment conferred increased resistance against UV and mitomycin C (MC) to ssb- mutants of Escherichia coli. The nucleotide (nt) sequence revealed that SSB consists of 175 amino acids (aa) and has an M(r) of 18,677. It shows 89% aa...
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