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Rapid and Sensitive Quantification of Borrelia burgdorferi -Infected Mouse Tissues by Continuous Fluorescent Monitoring of PCR

1 Apr 1999

Abstract excerpt

The quantity of Borrelia burgdorferi organisms in tissue samples is an important determinant for infection studies in the mouse model of Lyme disease. This report presents the development of a rapid and sensitive external-standard-based PCR assay for the absolute quantification of B. burgdorferi in mouse tissue samples. The assay uses a double-stranded DNA dye to continuously monitor product formation and in less...

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PMID: 10074514
DOI: 10.1128/jcm.37.4.987-992.1999

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