Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Semi-nested, multiplex polymerase chain reaction for detection of human malaria parasites and evidence of Plasmodium vivax infection in Equatorial Guinea.

The American journal of tropical medicine and hygiene - 1 Feb 1999

Rubio J M, Benito A, Roche J, Berzosa P J, García M L, Micó M, Edú M, Alvar J

Abstract excerpt

A semi-nested, multiplex polymerase chain reaction (PCR) based on the amplification of the sequences of the 18S small subunit ribosomal RNA (ssrRNA) gene was tested in a field trial in Equatorial Guinea (a hyperendemic focus of malaria in west central Africa). The method uses a primary PCR amplification reaction with a universal reverse primer and two forward primers specific for the genus Plasmodium and to...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Black People
Share to Malaria, Vivax

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Microscopy

Share to Plasmodium falciparum

Share to Plasmodium vivax

Share to RNA, Ribosomal, 18S

Share to Sensitivity and Specificity

Share to Templates, Genetic

Source

Identifiers

PMID: 10072133
DOI: 10.4269/ajtmh.1999.60.183

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Semi-nested, multiplex polymerase chain reaction for detection of human malaria parasites and evidence of Plasmodium vivax infection in Equatorial Guinea.PMID 10072133Read ↗

Use of real-time multiplex PCR, malaria rapid diagnostic test and microscopy to investigate the prevalence of Plasmodium species among febrile hospital patients in Sierra LeoneSemantic proximity Read ↗

Alternative polymerase chain reaction method to identify Plasmodium species in human blood samples: the semi-nested multiplex malaria PCR (SnM-PCR)Semantic proximity Read ↗

Development of a Polymerase Chain Reaction (PCR) method based on amplification of mitochondrial DNA to detect Plasmodium falciparum and Plasmodium vivaxSemantic proximity Read ↗

Human malaria diagnosis using a single-step direct-PCR based on the Plasmodium cytochrome oxidase III geneSemantic proximity Read ↗

Evaluation of three PCR-based diagnostic assays for detecting mixed Plasmodium infectionSemantic proximity Read ↗

A Comparative Analysis of PCR-Based Detection Methods for Avian MalariaSemantic proximity Read ↗

Detection and identification of human Plasmodium species with real-time quantitative nucleic acid sequence-based amplificationSemantic proximity Read ↗

Real-time PCR detection of mixed Plasmodium ovale curtisi and wallikeri infections in human and mosquito hostsSemantic proximity Read ↗

Real-time PCR assay in differentiating Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii infections in Orang Asli settlements in MalaysiaSemantic proximity Read ↗

Development of insulated isothermal PCR for rapid on-site malaria detectionSemantic proximity Read ↗

Detection of malaria parasites by nested PCR in south-eastern, Iran: Evidence of highly mixed infections in Chahbahar districtSemantic proximity Read ↗

A genus- and species-specific nested polymerase chain reaction malaria detection assay for epidemiologic studies.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.