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Evaluation of three PCR-based diagnostic assays for detecting mixed Plasmodium infection

31 Mar 2010

Abstract excerpt

BACKGROUND: One of the most commonly used molecular test for malaria diagnosis is the polymerase chain reaction (PCR)-based amplification of the 18S ribosomal DNA (rDNA) gene. Published diagnostic assays based on the 18S gene include the "gold standard" nested assay, semi-nested multiplex assay, and one tube multiplex assay. To our knowledge, no one has reported whether the two multiplex methods are better at...

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PMID: 20356398
DOI: 10.1186/1756-0500-3-88

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