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Development of insulated isothermal PCR for rapid on-site malaria detection

1 Mar 2016

Abstract excerpt

BACKGROUND: Detection of Plasmodium spp. is sometimes inconvenient especially in rural areas that are distant from a laboratory. In this study a portable diagnostic test of Plasmodium spp. was developed using insulated isothermal polymerase chain reaction (iiPCR) as an alternative approach to improve this situation. METHODS: A pair of universal primers and probe were designed to amplify and detect gene encoding...

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PMID: 26931146
DOI: 10.1186/s12936-016-1183-z

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