Article
Antibody analysis of the localisation, expression and stability of HlyD, the MFP component of the E. coli haemolysin translocator.
Molecular & general genetics : MGG - 1 Feb 1999
Pimenta A L, Young J, Holland I B, Blight M A
Abstract excerpt
HlyD has a single transmembrane domain (residues 59-80) and a large periplasmic domain, and is essential for the secretion of haemolysin from Escherichia coli. Using an antibody raised against HlyD, the protein was localised to the cell envelope by immunofluorescence and to the cytoplasmic membrane by sucrose gradient analysis. We have examined the stability of this protein in the presence and absence of other...
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