Article
Involvement of the C-terminal region of yeast proteinase B inhibitor 2 in its inhibitory action.
Journal of molecular biology - 26 Feb 1999
Kojima S, Deguchi M, Miura K
Abstract excerpt
Yeast proteinase B inhibitor 2 (YIB2), which is composed of 74 amino acid residues, is an unusual serine protease inhibitor, since it lacks disulfide bonds. To identify its reactive site for proteases, we constructed an expression system for a synthetic YIB2 gene and then attempted to change the inhibitory properties of YIB2 by amino acid replacements. The purified wild-type YIB2 inhibited the activity of...
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