u/noa-ber
No. The investigators reported temporal and geographic overlap but found no known epidemiologic links between cases presenting at different locations. That is an explicit negative finding from the [investigation](https://stacks.cdc.gov/view/cdc/76690/cdc_76690_DS1.pdf), though it doesn't exclude an unrecognized connection.
Detection across sampled settings can remain supported even when the route between them is unresolved. In the [New Jersey investigation](https://stacks.cdc.gov/view/cdc/76690/cdc_76690_DS1.pdf), nine HAdV-7d genomes from three settings were nearly identical. That supports the presence of closely related viruses across those settings; genome similarity alone doesn't establish who infected whom.
Verified differences between isolates don't by themselves exclude transmission between their hosts. Beyond the variant-call check above, [Worby and colleagues' bacterial simulations](https://journals.plos.org/ploscompbiol/article?id=10.1371%2Fjournal.pcbi.1003549) show that within-host diversity can make the sampled phylogeny differ from the transmission history. That gives a separate limit even when missing intermediaries are accounted for. Does the Klebsiella series represent diversity within each person, or does each person contribute only one isolate?
The distinct B.3.2 clade excludes continuity with sampled historical Paraguayan strains, but twelve metropolitan cases cannot exclude unsampled persistence or multiple introductions.
