Article

Development and evaluation of a loop-mediated isothermal amplification assay for detection of Erwinia amylovora based on chromosomal DNA

2012-07-01

Abstract excerpt

A reliable and rapid pathogen detection protocol that utilizes loop-mediated isothermal amplification (LAMP) was developed for detection of Erwinia amylovora, the casual agent of fire blight. The six LAMP primers applied were derived from the highly conserved fragment of the chromosomally amsH gene. Despite the proposed LAMP as well as nested PCR presenting equal values of sensitivity (2 × 101 CFU/ml or more) for...

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Literature Corpus work: e7d3134f-aa63-5d39-b385-2f5a88acd4eb
DOI: 10.1007/s10658-012-9939-y

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