Article
Sensitive and species-specific detection of Erwinia amylovora by polymerase chain reaction analysis.
Applied and environmental microbiology - 1 Nov 1992
Bereswill S, Pahl A, Bellemann P, Zeller W, Geider K
Abstract excerpt
Detection and identification of the fire blight pathogen, Erwinia amylovora, can be accurately done by polymerase chain reaction (PCR) analysis in less than 6 h. Two oligomers derived from a 29-kb plasmid which is common to all strains of E. amylovora were used to amplify a 0.9-kb fragment of the plasmid. By separation of the PCR products on agarose gel, this fragment wa specifically detected when E. amylovora...
Topics
- Base Sequence
- Erwinia
- Genetic Variation
- Geography
- Molecular Sequence Data
- Plant Diseases
- Plants
- Polymerase Chain Reaction
- Sensitivity and Specificity
- Species Specificity