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Selective detection of Pseudomonas syringae pv. tomato using dot blot hybridization and real-time PCR

2007-08-01

Abstract excerpt

Two rapid detection methods based on dot blot hybridization with a nonradioactive DNA probe and molecular beacon-PCR were developed for the specific detection of Pseudomonas syringae pv. tomato, the causal agent of bacterial speck of tomato. A 1378 bp DNA fragment (Acc. No. AM039892), obtained from the extension of a 255 bp fragment generated by a RAPD protocol, was used to find a suitable combination of primers s...

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Literature Corpus work
e5545f16-b8df-5e82-9701-90de92065a27
DOI
10.1111/j.1365-3059.2007.01612.x
Open publication

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Selective detection of Pseudomonas syringae pv. tomato using dot blot hybridization and real-time PCRDOI 10.1111/j.1365-3059.2007.01612.x
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