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Identification and in planta detection of Pseudomonas syringae pv. tomato using PCR amplification of hrpZ(Pst)

2005-01-01

Abstract excerpt

A rapid detection method based on PCR amplification of Pseudomonas syringae pv. tomato chromosomal sequences was developed. Primer design was based on the P. syringae DC3000 hrpZPst gene, which maps on a pathogenicity-associated operon of the hrp/hrc pathogenicity island. A 532 bp product corresponding to an internal fragment of hrpZPst was amplified from 50 isolates of P. syringae pv. tomato belonging to a geogra...

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Literature Corpus work
04906df9-2709-51c1-b757-55892cd91a26
DOI
10.1007/s10658-004-2734-7
Open publication

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