Article

Production of Antiserum to Recombinant Coat Protein for Detecting Lily mottle virus in Yunnan, China

2009-06-01

Abstract excerpt

Lily mottle virus (LMoV), Potyvirus genus, is very difficult to purify for the preparation of diagnostic antisera. The coat protein (CP) gene of LMoV was amplified by RT-PCR from infected plants and cloned into the prokaryotic pET-30a vector to generate the recombinant plasmid pET-CP. The resulting carboxy-terminal His-tagged CP was over-expressed in Escherichia coli BL 21 cells by Isopropyl-β- d-thiogalactoside (...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: dba02c61-e4ad-59ce-a93a-3d459279503e
DOI: 10.1111/j.1439-0434.2008.01491.x

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Production of Antiserum to Recombinant Coat Protein for Detecting Lily mottle virus in Yunnan, ChinaDOI 10.1111/j.1439-0434.2008.01491.xRead

Plasticity of the lettuce infectious yellows virus minor coat protein (CPm) in mediating the foregut retention and transmission of a chimeric CPm mutant by whitefly vectors.Semantic proximity Read

Plant-Derived Recombinant Vaccines against Zoonotic VirusesSemantic proximity Read

Molecular characterization of the CP gene and 3'UTR of Chilli veinal mottle virus from South and Southeast AsiaSemantic proximity Read

Development and application of a reverse transcription loop-mediated isothermal amplification combined with lateral flow dipstick for rapid and visual detection of Citrus leaf blotch virus in kiwifruitSemantic proximity Read

Serological Detection of Grapevine leafroll virus 2 Using an Antiserum Developed against the Recombinant Coat ProteinSemantic proximity Read

First Report of Milk Vetch Dwarf Virus Infecting Lily in KoreaSemantic proximity Read

Development and application of triple antibody sandwich enzyme-linked immunosorbent assays for begomovirus detection using monoclonal antibodies against Tomato yellow leaf curl Thailand virus.Semantic proximity Read

Genetic variability in the coat protein genes of Cymbidium mosaic virus isolates from orchids.Semantic proximity Read

First Report of Peanut Mottle Virus Infecting Peanut in Northeast ChinaSemantic proximity Read

Characterization of the Coat Protein Gene of Cymbidium mosaic virus Isolates from IndiaSemantic proximity Read

Polyclonal Antibodies to the Coat Protein of Carnation etched ring virus Expressed in Bacterial System: Production and Use in ImmunodiagnosisSemantic proximity Read

First Report of Pepper Mild Mottle Virus in Paris polyphylla var. yunnanensis in ChinaSemantic proximity Read